Ding-Wen (Roger) Chen
May 10, 2019
Homeostatic and regenerative regulation of stem cells by extracellular vesicles
Hematopoietic stem cells (HSCs) play a key role in the hematopoietic system, generating all mature blood and immune cells. However, the biology of how HSCs are maintained and regulated in physiological and pathological conditions remain only beginning to be understood, and require elucidation.
Extracellular vesicles (EVs) are membrane-enclosed vesicles secreted by cells into the extracellular space, carrying nucleic acid and protein cargo for delivery to target cells and downstream signaling. My research at the Kurre laboratory aims to improve our understanding of the biology involved in HSC regulation, particularly from the angle of EV-mediated signaling and its role in the paracrine regulation of HSCs by other cell types in the bone marrow microenvironment. To tackle this question, a novel transgenic animal model and advanced analytical techniques in cytometry, microscopy will be employed. My work will systematically resolve stromal and endothelial EV trafficking in the BM compartment to define EV contributions during HSC homeostasis and regenerative conditions. More broadly, insight gained will help with the development of novel therapeutic and treatment strategies that address currently unmet clinical needs in the treatment of bone marrow failure conditions.