Postdoctoral Fellow Opening
|(Ref Id: 23691)||[Email Posting] [Print Posting]|
|Date Posted||Jan 28, 2019|
|Faculty Member||Daria Babushok|
|Number of Slots||1|
|School||Perelman School of Medicine|
|Organization||DM-Hematology/Oncology Cancer Biology|
|Description||The Babushok laboratory seeks an enthusiastic and dedicated Ph.D. or M.D./Ph.D. scientist to join our laboratory as a Post-Doctoral Fellow at the Perelman School of Medicine University of Pennsylvania. The Babushok laboratory performs cutting-edge translational research at the interface of hematology, immunology and molecular genetics to interrogate the mechanisms of acquired and inherited bone marrow failure and the link between bone marrow failure and leukemia. We recently identified a critical connection between the development of clonal hematopoiesis in immune-mediated bone marrow failure called aplastic anemia and somatic loss of specific HLA class I alleles, shedding light on the mechanism of the autoimmune attack in this disease. Research opportunities are available investigating various facets of the immune mechanism of aplastic anemia. The studies will utilize a variety of cutting-edge proteomics and immunogenetic approaches, including mass spectrometry-based proteomic analyses of immune epitopes, next-generation sequencing analyses of HLA and TCR genes, use of genetic engineering technologies to create cell lines bearing designer TCR and HLA alleles, CRISPR-based cell culture models, cytotoxicity assays, RNA interference and pharmacologic inhibition of involved pathways using in vitro and murine models.|
Experience in immunology and immunogenetics, protein biochemistry, mass cytometry, flow cytometric analysis and sorting of cell populations, expertise with T cell functional assays, DNA construct design and cloning, transduction of primary and cultured cells, analysis of cellular proteins using immunoprecipitation, SDS-PAGE and Western blotting, analysis of gene expression using quantitative RT-PCR, and scientific knowledge and research experience in human immunology with a track record of publication in peer-reviewed journals. Experience with interpretation and analysis of TCR sequencing and next-generation sequencing data is a plus.
Ph.D. or M.D./Ph.D. and research experience in immunology, cell, and molecular biology, immunology, hematopoiesis, or a closely-related field. The candidate must be able to work independently as well as a member of a larger research team in a highly dynamic and collaborative work environment. The candidate must be able to troubleshoot technical difficulties, be willing to learn and optimize novel technologies, must maintain accurate and timely records, and must have excellent communication skills.
|Contact||Daria V. Babushok, MD PhD
Assistant Professor of Medicine and Pediatrics
Biomedical Research Building II/III, Room 808
421 Curie Boulevard
Philadelphia, PA 19104
Email: daria.babushok [at] uphs.upenn.edu